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Frequency: Quarterly
ISSN: 1608-4217 (Print)
ISSN: 2410-8561 (Online)
Research Article Open Access

ISOLATION AND CHARACTERIZATION OF HELICOBACTER PYLORI STRAINS FROM GASTRIC BIOPSIES OF ALGERIAN PATIENTS

Guetarni Hassina1 and Bensoltane Ahmed2
  • 1 University of Khemis Miliana, Algeria
  • 2 University of Oran Es-Senia, Algeria

Abstract

The objective of this study is to isolate and characterize strains Helicobacetr pylori from gastric biopsies by upper gastrointestinal endoscopy. After a rapid urease test, two Helicobacter pylori strains isolated from four patients on blood agar. After extraction of DNA from these strains from homogenates of gastric biopsies, quantification by real-time PCR was performed to determine Helicobacter pylori on the one hand and sensitivity to clarithromycin species. Melting curve analyzes showed that these two strains are sensitive to the antibiotic.

OnLine Journal of Biological Sciences
Volume 13 No. 2, 2013, 41-49

DOI: https://doi.org/10.3844/ojbsci.2013.41.49

Submitted On: 2 June 2013 Published On: 19 April 2013

How to Cite: Hassina, G. & Ahmed, B. (2013). ISOLATION AND CHARACTERIZATION OF HELICOBACTER PYLORI STRAINS FROM GASTRIC BIOPSIES OF ALGERIAN PATIENTS. OnLine Journal of Biological Sciences, 13(2), 41-49. https://doi.org/10.3844/ojbsci.2013.41.49

Open AccessCopyright: © 2013 Guetarni Hassina and Bensoltane Ahmed. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Keywords

  • Helicobacter Pylori
  • Culture
  • Urease
  • Real-Time PCR
  • Clarithromycin