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ISSN: 1608-4217 (Print)
ISSN: 2410-8561 (Online)
Research Article Open Access

 Association of High-Affinity FcεRI-β intron 2 for IgE Gene Polymorphism and Atopic Diseases in an Algerian Population

Dahbia Ines Dahmani1, Ikhlass Haj Salem2, Jamila Chakir2, Karima Sifi3, Sellami Abderrahim4, Mounir Zhary Bachtarzi5, Sara Malki1, Laila Rouabah6, Nore-Eddine Abadi3, Mehdioui Hacene1, Mohamed Bougrida1 and Mahmoud Rouabhia7
  • 1 Research Laboratory of Metabolic Diseases, Faculty of Medicine, University of Constantine-3, Algeria
  • 2 Centre de Recherche, Institut Universitaire de Cardiologie et de Pneumologie de Québec, Université Laval, Québec, Canada
  • 3 Research Laboratory of Biology and Molecular Genetics, Faculty of Medicine, University of Constantine-3, Algeria
  • 4 Laboratory of Expertise and Analysis of Sport Performance, University of Constantine 2, Algeria
  • 5 Clinique Privé D'allergologie Constantine, Algeria
  • 6 Laboratory of Cellular and Molecular Biology, Faculty of the Sciences, Constantine1 University, Algeria
  • 7 Oral Ecology Research Group, Faculty of Dentistry, Laval University, Quebec, QC, Canada

Abstract

Atopy is characterized by the production of a high level of IgE in response to common allergens. We examined the RsaI-in2 polymorphism of the FcεRI-β gene and its association with atopic asthma and comorbidity. The study population included 77 atopic asthmatics and 77 non-atopic, non-allergic and non-asthmatic controls. Atopic asthma was confirmed by the skin prick test and by IgE and interleukin IL-4 levels. RsaI-in2 polymorphism was determined by RFLP-PCR using the RsaI enzyme. A significant correlation was found between genotype BB and sensitization to house dust mites in the asthmatic subjects (p = 0.000). Results also reveal significant allele sharing in the subjects affected by eczema (p = 0.000) and allergic rhinitis (p = 0.01) during childhood. Furthermore, the subjects with heterozygous AB and homozygous BB genotypes displayed significant IgE levels > 1000 UI mL-1. IL-4 levels were also > 1000 pmol/ml for RsaI-in2 polymorphism genotype BB. Our findings show a strong association between genotype BB and the incidence of eczema and allergic rhinitis in childhood as well as increased IgE and IL-4 levels.

OnLine Journal of Biological Sciences
Volume 17 No. 4, 2017, 260-269

DOI: https://doi.org/10.3844/ojbsci.2017.260.269

Submitted On: 20 June 2017 Published On: 1 November 2017

How to Cite: Dahmani, D. I., Salem, I. H., Chakir, J., Sifi, K., Abderrahim, S., Bachtarzi, M. Z., Malki, S., Rouabah, L., Abadi, N., Hacene, M., Bougrida, M. & Rouabhia, M. (2017).  Association of High-Affinity FcεRI-β intron 2 for IgE Gene Polymorphism and Atopic Diseases in an Algerian Population. OnLine Journal of Biological Sciences, 17(4), 260-269. https://doi.org/10.3844/ojbsci.2017.260.269

Open AccessCopyright: © 2017 Dahbia Ines Dahmani, Ikhlass Haj Salem, Jamila Chakir, Karima Sifi, Sellami Abderrahim, Mounir Zhary Bachtarzi, Sara Malki, Laila Rouabah, Nore-Eddine Abadi, Mehdioui Hacene, Mohamed Bougrida and Mahmoud Rouabhia. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Keywords

  • Allergy
  • Asthma
  • FcεRI-β
  • IgE
  • IL-4
  • RsaI-in2 Polymorphism